AccuNGS - accurate sequencing

AccuNGS (Accurate Next Generation Sequencing) is a sequencing approach that maximizes accuracy and yield, while maintaining simplicity for both wet-lab protocols and computational analysis. It is extremely useful, for example, for sequencing of RNA or DNA virus populations that often maintain varying levels of genetic diversity.

Citation

The method preprint is available at Biorxiv.

Before you start

• Overview
• Downloading and installing AccuNGS

AccuNGS code components

• Base calling
  • Base calling stage
  • Output format
  • Example: sample data
• Variant calling
  • Prerequisites
  • Input
  • Output
  • Example
• Haplotype inference
  • Prerequisites
  • Finding pairs of linked loci
  • Linked pairs to haplotypes
• Using barcoded templates